Few stop to consider the consequences of their daily ablutions, the washing of clothes, the watering of lawns, and the flush of a toilet. However, wastewater treatment--one of the cornerstones of modern civilization--is the largest microbially-mediated biotechnology process on the planet. When it works, it is a microbial symphony in tune with humanity. When it fails, the consequences can be dire. Researchers from the U.S. Department of Energy Joint Genome Institute (DOE JGI) and collaborators at the University of Wisconsin-Madison, and the Advanced Wastewater Management Centre, University of Queensland, Australia, have published the first metagenomic study of an activated sludge wastewater treatment process. The research appeared online in the September 24 edition of the journal Nature Biotechnology (http://www.nature.com/nbt/journal/vaop/ncurrent/abs/nbt1247.html).