
Auto-fluorescence of lignin in a root-hypocotyl cross-section of the nst1-1 nst3-1 double mutant shows a conspicuous absence of lignified secondary xylem. Wood is formed from secondary xylem tissue consisting of cells with a heavily thickened secondary cell wall that is enriched in lignin and cellulose. In contrast, primary cell walls are composed mainly of polysaccharides (including cellulose), but contain very little lignin. Wood formation occurs through a complex series of steps involving cell division and expansion and the biosynthesis of lignin and cellulose. In addition, heartwood (darker wood at the center of the trunk) forms in many tree species through a highly regulated process of programmed cell death.


